contextual fear conditioning paradigm chambers  (Med Associates Inc)

Journal: Nature Communications

Article Title: Genetically- and spatially-defined basolateral amygdala neurons control food consumption and social interaction

doi: 10.1038/s41467-024-50889-7

Figure Lengend Snippet: A Scheme of contextual fear conditioning assay (CFC). B Peri-stimulus time histogram (PSTH) illustrating inferred spike trains from calcium responses during the footshock session of CFC in Lypd1 (top) and Etv1 (bottom) mice. Trial numbers are neuron numbers multiplied by three footshocks, sorted by footshock response score (SRC). C Histogram of SRC in BLA Lypd1 (top) and BLA Etv1 (bottom) mice showing negative correlation (anti-footshock, purple), positive correlation (pro-footshock, orange), and neutral (gray). ( N = 179 and 211 neurons recorded from 7 and 6 mice, BLA Lypd1 , BLA Etv1 respectively). D Scatter plots show the relationship between freezing frequency during fear retrieval and percentages of pro-footshock neurons in fear acquisition for Lypd1 (top) and Etv1 (bottom) mice ( n = 5 mice each, the corresponding R 2 and p -values from the two-sided test. The plots on the right show the binarized freezing traces for each mouse. E Schemes of social interaction assays (two chamber or round chamber with conspecific). F Response maps of three example neurons (pro-social, anti-social, neutral). G , H Averaged firing rate heatmaps to social distance during the social interaction assay in all neurons ( G ) or significant neurons determined with a permutation test ( H ) ( N = 64, 148, and 121 neurons recorded from 7, 6, and 5 of BLA Lypd1 , BLA Etv1 and BLA Rspo2 mice, respectively). I Violin plots of peak firing distances (significant neurons: orange; all neurons: gray). J – L Percentages of neurons with peak firing rates in pro-social, neutral, and anti-social areas for BLA Lypd1 ( J ), BLA Etv1 ( K ) and BLA Rspo2 ( L ) populations. Significance at the 2.5% level with null distribution (shuffled) is indicated (two-tailed test, mean ± SD). Significant cell numbers are for Lypd1; n = 4,8,17 and Etv1; 40,10,17 and Rspo2; 9,16,11 from the pro-food, neutral, and anti-food areas, respectively. Data are presented as mean values SD ( J – L ). * p < 0.05, ** p < 0.001, **** p < 0.0001, with all t tests being two-tailed.

Article Snippet: On day 1, mice were placed into a contextual fear conditioning chamber (Med Associates) while bilaterally connected to optic fiber cables and received three foot-shocks (0.75 mA for 2 s) at the 198-s, 278-s and 358-s time points.

Techniques: Two Tailed Test

Journal: Nature Communications

Article Title: Genetically- and spatially-defined basolateral amygdala neurons control food consumption and social interaction

doi: 10.1038/s41467-024-50889-7

Figure Lengend Snippet: A Schemes of AAV injections and optic-fiber placements above ChR2- and eNpHR-expressing BLAs; modified from Allen Mouse Brain Atlas, mouse.brain-map.org. B Representative images of ChR2-eYFP expression in Rspo2, Etv1 and Lypd1-Cre mice with optic fiber locations. C Left: Scheme of optogenetic activation during the free-feeding assay. Right: Food intake during optogenetic activation of three BLA populations compared to light-off epochs and photostimulated controls. Lypd1: n = 14 (ChR2) and 9 mice (YFP) per group with a two-tailed paired t test, t ( 13 ) = 2.457, p = 0.0288 within ChR2 (on versus off). For ChR2-On versus YFP-On: two-tailed unpaired t test, t (21) = 3.4, p = 0.0027). Etv1: n = 11 (ChR2) and 7 mice (YFP) per group with a two-tailed paired t test, t ( 9 ) = 2.492, p = 0.0343 within ChR2 (on versus off)). Rspo2: n = 18 (ChR2) and 9 mice (YFP) per group with Wilcoxon matched pairs signed rank test, p = 0.0023 within ChR2 (on versus off) group. For ChR2-On versus YFP-On: Kolmogorov–Smirnov test, p = 0.0226. D Left: Scheme of optogenetic inhibition. Right: Food intake during optogenetic inhibition of three BLA populations compared to light-off epochs and photostimulated controls. Lypd1: n = 9 (eNpHR 3.0) and 11 mice (mCherry) per group with two-tailed paired t test, t ( 8 ) = 2.771, p = 0.0243 within eNpHR 3.0 (on versus off) group). Etv1: n = 8 (eNpHR 3.0) and 7 mice (mCherry) per group. Rspo2: n = 13 (eNpHR 3.0) and 10 mice (mCherry) per group. E Left: Scheme of conditioned-place preference experiment. Right: Preference index (cumulative time % in paired chamber—cumulative time % in unpaired chamber) of cohorts of mice before (pre) and after (post) conditioning. Lypd1: n = 13 (ChR2) and 7 mice (YFP) per group; paired t test, t (12) = 4.528, p = 0.0007 within ChR2 group (pretest versus posttest), Etv1: n = 11 (ChR2) and 9 mice (YFP) per group; paired t test, t (10) = 3.273, p = 0.0084 within ChR2 group (pretest versus posttest). Rspo2: n = 8 mice (ChR2 and YFP) per group; two-tailed paired t test, t (7) = 2.695, p = 0.0308, within ChR2 group (pretest versus posttest). All box-and-whisker plots show whiskers down to the minimum and up to the maximum value and boxes with center (median) and bounds of the box (75th and 25th percentile). * p < 0.05, ** p < 0.01, *** p < 0.001. All t tests used two-tailed.

Article Snippet: On day 1, mice were placed into a contextual fear conditioning chamber (Med Associates) while bilaterally connected to optic fiber cables and received three foot-shocks (0.75 mA for 2 s) at the 198-s, 278-s and 358-s time points.

Techniques: Expressing, Modification, Activation Assay, Feeding Assay, Two Tailed Test, Inhibition, Conditioned Place Preference, Whisker Assay

Journal: Nature Communications

Article Title: Genetically- and spatially-defined basolateral amygdala neurons control food consumption and social interaction

doi: 10.1038/s41467-024-50889-7

Figure Lengend Snippet: A Left: Scheme of contextual fear conditioning with photostimulation; Day 1: 3 footshocks (0.75 mA) paired with light on. Freezing measured on Day 2 (fear recall). Right: Freezing behavior (%) on Day 2 with photostimulation of two BLA populations compared to controls. Lypd1: n = 7 (ChR2) and 5 mice (YFP) per group; Kolmogorov–Smirnov test, p = 0.0152), Etv1: n = 6 (ChR2) and 7 (YFP) mice per group; Kolmogorov–Smirnov test, P = 0.9254. B Left: Scheme of contextual fear conditioning with photoinhibition; Right: Freezing behavior (%) on day 2 combined with photoinhibition of two BLA populations compared to controls. Lypd1: n = 12 (eNpHR 3.0) and 8 (mcherry) mice per group; unpaired t test, p = 0.0204, * p < 0.05. Etv1 groups: n = 11 (eNpHR 3.0) and 7 (mcherry) mice per group; unpaired t test, p = 0.0096. C Schemes of social interaction assay with photoactivation; Right: Cumulative duration in the social zone (%) combined with photoactivation of three BLA populations compared to light-off epochs and controls. Lypd1; n = 8 (ChR2) and 6 mice (YFP) per group; two-tailed paired t test, t (7) = 2.307, p = 0.0544 within ChR2 group (on versus off); Etv1; n = 12 (ChR2) and 8 mice (YFP) per group; paired t test, t (11) = 3.785, p = 0.0030, within ChR2 group (on versus off); Rspo2; n = 6 (ChR2) and 4 mice (YFP) per group; paired t test, t (7) = 0.6806, p = 0.5180, within ChR2 group (on versus off). D Schemes of social interaction assay with photoinhibition; Right: Cumulative duration in the social zone (%) combined with photoinhibition of three BLA populations compared to light-off epochs and controls. Lypd1; n = 5 (eNpHR3.0) and 4 mice (mcherry) per group; Wilcoxon matched-pairs signed rank test, p = >0.9999, within eNpHR 3.0 group (on versus off); Etv1; n = 11 (eNpHR3.0) and 5 mice (mcherry) per group; paired t test, t (10) = 3.19, p = 0.0097, within eNpHR 3.0 group (on versus off); Rspo2; n = 9 (eNpHR3.0) mice, Wilcoxon matched-pairs signed rank test, p = 0.6250, within eNpHR 3.0 group (on versus off). All box-and-whisker plots show whiskers down to the minimum and up to the maximum value and boxes with center (median) and bounds of the box (75th and 25th percentile with all t tests being two-tailed. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: On day 1, mice were placed into a contextual fear conditioning chamber (Med Associates) while bilaterally connected to optic fiber cables and received three foot-shocks (0.75 mA for 2 s) at the 198-s, 278-s and 358-s time points.

Techniques: Two Tailed Test, Whisker Assay

Journal: Nature Communications

Article Title: Genetically- and spatially-defined basolateral amygdala neurons control food consumption and social interaction

doi: 10.1038/s41467-024-50889-7

Figure Lengend Snippet: Alphabetical list of abbreviations

Article Snippet: On day 1, mice were placed into a contextual fear conditioning chamber (Med Associates) while bilaterally connected to optic fiber cables and received three foot-shocks (0.75 mA for 2 s) at the 198-s, 278-s and 358-s time points.

Techniques: Conditioned Place Preference, Expressing, Saline, In Situ Hybridization, Standard Deviation

Journal: STAR Protocols

Article Title: Protocol for remapping of place cells in disease mouse models

doi: 10.1016/j.xpro.2021.100759

Figure Lengend Snippet:

Article Snippet: Chambers for Contextual Fear Conditioning , Med Associates , https://www.med-associates.com/.

Techniques: Knock-In, Software